The Lee Covid test centre on the northside of Cork city closed today after 19 months.
The centre opened in September 2020 and carried out an estimated 290,675 swabs.
Head of Health and Wellbeing with Cork Community Healthcare Gabrielle O'Keeffe thanked staff for their work.
"Most people in Cork have attended this centre at some stage in the last 19 months, and they are aware of how staff there have carried out their work in the most professional and caring way," she said.
There is now one test centre for Covid-19 in Cork city, on the South Douglas Road, with appointments available seven days a week via the self-referral portal on HSE.ie or GP referral.
The HSE said it is now adapting its testing and tracing programme in response to the significantly reduced prevalence and impact of Covid-19 in 2022, while making sure that both the infrastructure and staff are available if testing needs to scale back up quickly.
A spokesperson said: "We expect that all testing centres will close later this year, once the public health advice changes for population testing.
"PCR testing will remain available for those who need it to access therapeutic treatments, or as part of their clinical treatment."
Staff at the Lee Covid test centre have been invited to apply for other suitable positions within Cork Kerry Community Healthcare Service.
