Pilots at Emerald Airlines, the carrier which holds the regional service franchise for Aer Lingus, have served notice of 24-hour work stoppage to take place ahead of the August bank holiday weekend.
The pilots are members of IALPA, a branch of the Fórsa trade union.
Fórsa issued notice of the stoppage today, saying it will start at midnight on Thursday 3 August and will end at midnight Friday 4 August.
Emerald Airlines operates regional flights, including the Dublin to Donegal service on behalf of Aer Lingus and UK regional routes trading as Aer Lingus Regional.
The industrial action will include pickets at Emerald Airlines offices and at the Dublin Airport roundabout, and follows the escalation of a work-to-rule action by pilots earlier this week.
The union said the industrial action is in response to the airline's failure to engage with IALPA to form a collective labour agreement for pilots employed at the carrier.
Emerald pilots have been engaged in a strict work-to-rule since June 24.
The action includes a refusal to work overtime or out-of-duty hours, while pilots remain uncontactable by the company outside of working hours, and are not accepting changes to their rosters with fewer than 24 hours' notice.