The Irish Payment Services Organisation has said that all Laser bank cards have now been replaced with Visa and MasterCard debit cards.
The operation of Laser card transactions will cease at midnight on Friday 28 February.
IPSO said that after that date, retailers who try to process Laser card transactions will receive an error message only.
It said that retailers who currently accept Laser cards for subscriptions, ongoing payments for policies or recurring transactions should make arrangements with their customers to obtain an alternative payment method.
The last Laser file transmission will be processed almost 18 years to the day since the very first Laser transaction in 1996.
Laser cards reached their highest number in 2011 when there were more than 3 million active Laser debit cards in the market.