The Eurovision Song Contest final features the 20 successful nations from the week's two semi-finals, as well as the so-called big five of the UK, France, Germany, Italy and Spain.
Host nation the Netherlands will also compete in the showpiece event.
Cyprus’ Elena Tsagrinou will perform the track El Diablo in the opening slot, while San Marino's entry Senhit will close the show with their song Adrenalina.
James Newman, the older brother of pop star John Newman, is representing the UK and will perform his song Embers, an upbeat dance-pop offering inspired by the end of lockdown, ninth in the running order, in front of a crowd of 3,500 fans at the Ahoy arena.
Ireland's Lesley Roy missed out on a place in the final after performing her song Maps in the first semi-final on Tuesday night.
The contest has been disrupted by a number of positive Covid-19 tests among the delegations.
Iceland, one of the favourites to win, will compete in the final despite its entry, Daoi og Gagnamagnio, forced to pull out of performing live after a positive test.
The quirky dance-pop band had been due to perform their song 10 Years but will now compete using their rehearsal performance, recorded on the stage on May 13.
Reigning Eurovision Song Contest champion Duncan Laurence was also due to perform but pulled out after testing positive and developing mild symptoms.
Watch The Eurovision Song Contest final on RTÉ One and RTÉ Player, as well as coverage on RTÉ 2fm from 8pm.
EUROVISION SONG CONTEST GRAND FINAL 2021 FULL LINE-UP
Song: El Diablo (The Devil)
Composers/Lyricists: Thomas Stengaard, Jimmy 'Joker' Thörnfeldt, Laurell Barker, Oxa
Performer: Elena Tsagrinou
Composer: Kledi Bahiti / Lyricist: Olti Curri
Performer: Anxhela Peristeri
Song: Set Me Free
Composers/Lyricists: Amit Mordechay, Ido Netzer, Noam Zlatin, Ron Carmi
Performer: Eden Alene
Song: The Wrong Place
Composers/Lyricists: Alex Callier, Charlotte Foret
Song: Russian Woman
Composers: Ori Avni, Ori Kaplan Lyricist: Manizha Sanghin
Song: Je Me Casse
Composers/Lyricists: Malin Christin, Amanuel Dermont, Nicklas Eklund, Pete Barringer
Song: Love Is On My Side
Composer/Lyricist: Pedro Tatanka
Performer: The Black Mamba
Song: Loco Loco
Composers: Nemanja Antonic, Darko Dimitrov Lyricist: Sanja Vucic
9. UNITED KINGDOM
Composers/Lyricists: James Newman, Conor Blake, Danny Shah, Tom Hollings, Samuel Brennan
Performer: James Newman
Song: Last Dance
Composers: Dimitris Kontópoulos, Arcade Lyricists: Dimitris Kontópoulos, Arcade, Sharon Vaughn
Song: Tout L'Univers (The whole universe)
Composers: Wouter Hardy, Nina Sampermans, Gjon Muharremaj, Xavier Michel Lyricists: Wouter Hardy, Nina Sampermans, Gjon Muharremaj
Perfromer: Gjon’s Tears
Song: Ten Years
Composer/Lyricist: Daði Freyr Pétursson
Performer: Daði og Gagnamagnið
Song: Voy A Quedarme (I’m going to stay)
Composers/Lyricists: Blas Cantó, Leroy Sánchez, Daniel Ortega, Dan Hammond
Performers: Blas Cantó
Composers: Dimitris Kontopoulos, Philipp Kirkorov Lyricists: Mikhail Gutseriev, Sharon Vaughn
Performer: Natalia Gordienko
Song: I Don't Feel Hate
Composers/Lyricists: Jendrik Sigwart, Christoph Oswald
Song: Dark Side
Composers/Lyricists: Aleksi Kaunisvesi, Joonas Porko, Joel Hokka, Niko Moilanen, Olli Matela
Performer: Blind Channel
Song: Growing Up Is Getting Old
Composers/Lyricists: Victoria Georgieva, Maya Nalani, Oliver Björkvall, Helena Larsson
Composers: Vaidotas Valiukevicius, Mantas Banišauskas, Robertas Baranauskas, Laisvunas Cernovas, Ilkka Wirtanen Lyricists: Vaidotas Valiukevicius, Mantas Banišauskas, Kalle Lindroth
Performer: The Roop
Song: Shum (Noise)
Composers: Taras Shevchenko, Kateryna Pavlenko Lyricist: Kateryna Pavlenko
Song: Voilá (Here)
Composers: Barbara Pravi, Igit Lyricists: Barbara Pravi, Igit, Lili Poe
Performer: Barbara Pravi
Song: Mata Hari
Composers/Lyricists: Amy van der Wel, Luuk van Beers, Josh Earl, Tony Cornelissen
Song: Fallen Angel
Composer: Andreas Haukeland Lyricists: Andreas Haukeland, Emelie Hollow, Mathias Haukeland
Song: Birth Of A New Age
Composers: Jeangu Macrooy, P.L. Perquin Lyricist: Jeangu Macrooy
Performer: Jeangu Macrooy
Song: Zitti E Buoni (Shut up and behave)
Composers/Lyricists: Damiano David, Victoria DeAngelis, Thomas Raggi, Ethan Torchio
Composers/Lyricists: Joy Deb, Linnea Deb, Jimmy ‘Joker’ Thörnfeldt, Anderz Wrethov
26. SAN MARINO
Composers/Lyricists: Thomas Stengaard, Joy Deb, Linnea Deb, Jimmy Thörnfeldt, Kenny Silverdique, Suzi Pancenkov, Malou Linn Eloise Ruotsalainen, Chanel Tukia, Senhit Zadik Zadik, Tramar Dillard (Flo Rida) Performer: Senhit feat. Flo Rida