More than £52 million has been raised during Comic Relief 2021, which was aired on BBC One on Friday night.
The show saw the debut British broadcast of Paul Mescal, Daisy Edgar-Jones and Andrew Scott perform a sketch based on a crossover of Fleabag and Normal People.
The skit was first broadcast as part of RTÉ Does Comic Relief last June.
The charity event, which was filmed live in front of a virtual audience, featured a plethora of celebrity cameos and fundraising appeals.
Justin Bieber gave an exclusive performance of his song Hold On during the show.
Keira Knightley, Carey Mulligan, Michael Sheen and Jodie Whittaker starred in a joke film trailer for 2020 The Movie during the programme.
Host Alesha Dixon said at the end of the programme: "You have made an incredible difference to the lives of people here in the UK and across the world.
"Your generosity tonight will create lasting change for those who need our support now more than ever."
Her fellow presenter Sir Lenny Henry said: "After a year of lockdowns, we hope we have given you laughter and joy."
The programme was also hosted by television presenters Paddy McGuinness and Davina McCall, and actor David Tennant.
The fundraising total for Comic Relief will be updated again at a later stage once all the donations have been counted.
The Duke of Cambridge made an appearance in the programme to thank viewers for their "compassion and generosity" in raising millions for good causes during last year's Big Night In television fundraiser, which generated funds for Comic Relief.
Daniel Craig also featured in a sketch as James Bond alongside Catherine Tate's foul-mouthed character Nan.
Comedians Andi Osho, Alex Brooker, Jennifer Saunders, Jayde Adams, and Caroline Quentin performed opera favourite Nessun Dorma, after being coached by soprano Charlotte Church.
Tonight for @comicrelief this gang will be singin Opera. We are all marvellous. And I am assured that even trained opera singers couldn't make the noise that’s coming out of throat. #ComicRelief pic.twitter.com/1xFtcPBSKA— jennifer saunders (@ferrifrump) March 19, 2021
During the programme, comedian and children’s author David Walliams joined Lenny Henry to meet schoolchildren, who were discussing the recyclable red nose being sold for Red Nose Day.
Oscar-winning actress Olivia Colman, James Corden and Idris Elba, comedian Matt Lucas and singer Gary Barlow were among the other stars to feature in the fundraiser.