Avengers Assemble is set to become the first movie to make $200 million at the box office in a single weekend in cinemas in North America.
The film, based on Marvel Comics superhero characters, easily passed the previous first weekend record of $169.2 million, which was set last year by the final instalment of the Harry Potter series.
The Joss Whedon-directed film also had the biggest-ever Saturday gross, earning $69.7 million. With $441.5 million already made in other countries outside America since last week, its worldwide box office total climbed to $641.8 million.
Avengers Assemble is set to become one of only a handful of movies to gross $1 billion worldwide and a spokesman for Disney said: "It's been a fantastic weekend on every single front."