Pierre-Emerick Aubameyang has been axed from Chelsea's Champions League squad and there was also no place for January signing Benoit Badiashile, but British record transfer Enzo Fernandez was included.
Mykhailo Mudryk, who joined for an initial fee of €70million from Shakhtar Donetsk, and loan signing Joao Felix were also included in the 25-man list named.
The London club, who visit Borussia Dortmund in their last-16 first leg tie on 15 February, were only allowed to register a maximum of three new players for the knockout stage.
Aubameyang has failed to impress since joining the club from Barcelona in September, scoring only one Premier League goal in 11 appearances.
His last Premier League start was in November and he was substituted in a 1-0 defeat by Manchester City in early January after having come off the bench.
Chelsea manager Graham Potter acknowledged on Thursday that finding the right blend between their expensive influx of talent and the existing squad would be challenge.
Badiashile joined in a big-money deal from Monaco in early January but was left out with Chelsea seemingly well-stocked at centre back with Thiago Silva, Trevoh Chalobah, Wesley Fofana and Kalidou Koulibaly all included.
All clubs are limited to a 25-man squad and Chelsea used 24 players in the group stage, with only one of them - Jorginho - leaving the club in the January transfer window.
Teams can also include an unlimited number of B List players aged 21 or younger with two years' training at their club.