Premier League players in tier four areas in England will be tested for Covid-19 twice a week as soon as it is practically possible to do so.
Players in the top flight have been tested on a weekly basis since the start of the 2020-21 season.
But testing at clubs in tier four areas will now revert to the level used during 'Project Restart' in the summer.
The increased testing will be widened out to clubs in lower-tier areas from next month, it is understood.
The UK government placed large parts of south-east and eastern England into tier four over fears of a new strain of the virus which is believed to be more transmissible.
The Premier League clubs currently in tier four are Arsenal, Chelsea, Crystal Palace, Fulham, Tottenham and West Ham.
The Premier League had said when it cut testing back to once a week at the start of the new season that it would keep testing levels under review.
The league's latest figures were released on Monday night, and showed there were seven positive cases out of 1,569 players and club staff tested.