Leyton Orient's Carabao Cup tie with Tottenham is in doubt after a number of players at the Sky Bet League Two club tested positive for coronavirus.
The O’s were set to host the Premier League side in the third round on Tuesday night, but have closed their Breyer Group Stadium and training ground until further notice.
"Following our game on Saturday 19th September, Covid-19 testing was conducted on all of our first-team playing squad," a club statement from Leyton Orient read.
"Today (Monday 21st) we received formal confirmation which shows that a number of our first-team squad have tested positive for Covid-19.
"We have informed the EFL and Tottenham Hotspur and will make a further announcement regarding Tuesday’s match shortly."
EFL clubs no longer have to conduct mandatory tests on their players and staff for Covid-19 on a weekly basis, as is still the case in the Premier League.
But, ahead of the midweek tie with Spurs, Orient tested their squad and staff.
The club statement continued: "We have also informed Mansfield Town, Plymouth Argyle and Oldham Athletic, our three most recent opponents, prior to the public release of this information.
"Leyton Orient’s priority is the health and well-being of its players and staff. Those who have tested positive will now strictly follow government self-isolation guidelines.
"The Breyer Group Stadium and training ground facilities will be closed until further notice."
There is believed to be no protocol in the EFL competition regulations for this eventuality, in terms of whether the match should be forfeited or rescheduled.
The medical team at the EFL is understood to be liaising with club medics and Public Health England over next steps.
O’s boss Ross Embleton was due to speak at a press conference on Zoom at 3pm to preview the game with Spurs, but that has now been cancelled.
The cup tie with Tottenham was an emotional fixture for the two clubs given the connection of the late Justin Edinburgh, who died last June at the age of 49 following a cardiac arrest.
Edinburgh won the FA Cup and League Cup at Spurs before he turned his hand to management and guided the O’s to the Vanarama National League title in April 2019.
Harry Kane also scored his first professional goal while on loan at Brisbane Road from Spurs in 2011 and the relationship has lasted, with Kane sponsoring Orient's three kits this season.
Given Tottenham’s packed fixture schedule, with a Europa League tie on Thursday in Macedonia and potentially another next week if they triumph at Shkendija, the Premier League side have little wriggle room to rearrange the Carabao Cup third-round tie.
The fourth round of the tournament is due to take place the week commencing September 28, with the winner of the Breyer Group Stadium clash drawn to host Chelsea or Barnsley.