Ciaran Clark will miss the Republic of Ireland's Nations League showdown with Wales after picking up an injury in training.
The 29-year-old defender suffered a thigh problem ahead of the game with Wales and will return to his club, Newcastle, to be assessed.
A statement issued via the Newcastle's website said: "The club has been informed by the Football Association of Ireland that Ciaran Clark will not be fit for his country's UEFA Nations League game against Wales on Tuesday evening.
"The Newcastle defender suffered a thigh injury during training ahead of the match. He will be assessed further on his return to Newcastle later this week."
Calum O'Dowda had already been ruled out of tonight's game after suffering a dizzy spell during Ireland's 0-0 draw with Denmark on Saturday.
Clark was an unused substitute for his country's draw with Denmark.
He has started five of the Magpies' eight league games to date this season, but was named only among the substitutes for the 3-2 defeat at Manchester United in their last outing.
Follow Republic of Ireland v Wales with our live blog on RTÉ Sport Online and the News Now App and listen live on 2fm's Game On from 7pm