Four Premiership Rugby players and one club staff member have returned positive results in the latest round of coronavirus tests, the league has said.
In the fourth week of screening, 846 players and staff from England's elite clubs took part and those who tested positive have gone into isolation.
"Premiership Rugby can (on Wednesday) confirm that on Monday, July 27, 846 players and club staff were tested as part of the PCR Covid-19 screening programme," the league said.
"Of these, five people have tested positive. Of those five, four were players and one was a member of staff.
"Those who have tested positive and their close contacts will now isolate and be assessed in line with the Public Health England-agreed guidelines.
"Premiership Rugby and the RFU are providing this aggregated information for competition integrity and transparency.
"No specific details as to clubs or individuals will be provided. The number of positive results will be made public after each round of testing."
Premiership Rugby said that in the first round of screening on July 6 there were a total of 10 positive cases out of 804 tests.
Week two (commencing July 13) revealed a total of nine positive cases out of 856 tests and two players were shown to have coronavirus after 896 tests were carried out in the third round of screening (on July 20).
There have been no positive cases after two rounds of testing at the Irish provinces ahead of the scheduled resumption of the Pro14 next month.