Ireland will be without British and Irish Lions captain Paul O'Connell and hooker Rory Best for their tour of New Zealand and Australia after the pair were ruled out through injury.
Munster lock O'Connell was selected in Declan Kidney's initial 33-man party despite receiving intravenous antibiotics for a groin injury at a Cork hospital.
O'Connell, 30, suffered the injury during Ireland's final RBS 6 Nations game against Scotland and has failed to recover in time to tour, with Ulster's Ed O'Donoghue being called up as his replacement.
Best had missed most of the season after undergoing surgery on a serious neck injury last summer, but he returned ahead of schedule to play a part in the Six Nations.
But an ankle injury has also ruled the 27-year-old out and he is replaced by Leinster's John Fogarty.
The news robs Ireland of valuable experience, with back-rowers Stephen Ferris and Denis Leamy and back Luke Fitzgerald already missing from the trip down under.
Ireland prepare for their trip with a clash against Barbarians at Thomond Park in a week's time before meeting New Zealand in New Plymouth on 12 June.
The team is due to face New Zealand Maori on 18 June before rounding off the trip with a Test against Australia in Brisbane on 26 June.