Fine Gael TD Fergus O'Dowd has made a complaint to the Dáil ethics watchdog about Fianna Fáil's Brexit spokesperson Lisa Chambers.
The development follows a report in the Sunday Independent which claimed Ms Chambers sat in the seat of and voted on behalf of Fianna Fáil's Timmy Dooley on seven occasions on 17 January this year.
Mr Dooley has said he was in the Dáil chamber at the time.
Ms Chambers has said the incident was "entirely normal" as Mr Dooley was present in the Chamber.
Last week it emerged the Dáil Committee on Members Interests is to issue a warning to Ms Chambers after a separate incident in which she voted once for another Fianna Fáil TD, Dara Calleary, in October this year when he was outside the chamber.
The warning is expected to say that a repetition of the contravention of the rules of the Oireachtas could lead to a finding that Ms Chambers had acted "recklessly or intentionally".
Mr O'Dowd made his complaint to the Committee on Members Interests today.
The complaint states: "I would bring to the Committee's attention a radio interview given by Deputy Chambers to RTÉ's Morning Ireland programme on the 21st of October this year in the context of the complaint in relation to voting for Deputy Calleary.
"During that interview Deputy Chambers indicated that this was first time she had ever voted for another Deputy. That clearly was not the case."
In response to the complaint by Mr O'Dowd, a spokesman for Fianna Fáil said that if the Committee of Members Interests decide to pursue "this vexatious" complaint the committee was in for a very "busy period".
He added there were frequent examples of Fine Gael TDs voting for one another in exactly the same circumstances.
Following the report in the Sunday Independent yesterday, Ms Chambers told RTÉ that her colleague Timmy Dooley had confirmed that he was in the Dáil chamber at the time of the vote, and that it was "entirely normal" for her to be sitting in Mr Dooley's seat.
She added that this circumstance was no different to the multiple Government ministers and TDs who had votes recorded for them while they were not in their seats and while elsewhere in the Dáil chamber.
She said: "On the day in question you can see many other TDs were in different seats, this was entirely normal and people moved throughout the voting block, so I am unsure why I am being singled out in this regard."