A Minister of State at the Department of Education has said she has witnessed sexist behaviour in the Dáil.
Speaking on RTÉ's Drivetime, Mary Mitchell O'Connor said women in the Dáil are talked over and cut off from answering questions.
Ms Mitchell O'Connor said she believes it is a gender issue more so than one of bullying, adding that women are "ignored."
"I have seen sexism, I have seen macho behaviour," she said.
"I have been very uncomfortable in Dáil Éireann, as have many of the women in the Dáil. Shouting and roaring, cutting you off before you answer a question, be it in the Dáil or in a committee.
"I think it is gender issue, I think it happens more to women. Women are talked over, they're ignored, they're not allowed to answer questions, that's the way I see it," she added.
Ms Mitchell O'Connor added that she is especially concerned for the possible consequences of children seeing this kind of behaviour in the Dáil.
Earlier, the Taoiseach said that systemic bullying and harassment can never be tolerated in any workplace.
Leo Varadkar said he was encouraged that more people are telling their stories because he believes the culture can be changed here over time.
He urged anyone in Leinster House who felt they had been mistreated to come forward.
Yesterday Ceann Comhairle Seán Ó Fearghaíl said that he is "anxious to ensure that proper policies are in place" for tackling any sexual misconduct.
Mr Ó Fearghaíll told RTÉ News he had "no specific concern", but felt that political parties "may wish to similarly study the situation."
He is to raise the matter at the next meeting of the Houses of the Oireachtas Commission, which is the body responsible for running both the Dáil and the Seanad.
As well as serving as Ceann Comhairle, Mr Ó Fearghaíl is also the Chair of that commission.