The Gay and Lesbian Equality Network (Glen) has been directed by the Charities Regulator to provide specific information including financial reports, details of staff credit cards and the report of an independent auditor, RTÉ News has learned.
The direction stretches back three years to 2014.
The Charities Regulator is looking for details of all credit cards used by board members, staff and volunteers and any expenditure on them for 2014, 2015 and 2016.
It has also requested details of authorisations for the cards.
In addition, it wants to see board papers and financial reports along with any reports engaged by named accountants.
The HSE has said that it is currently in communication with Glen regarding funding for 2017.
In a statement, the charity said it made a voluntary disclosure to the Regulator following a review of its corporate governance procedures.
It said that it is fully cooperating with the Regulator and will continue to work towards implementing best practice in all areas of governance and fiduciary duty.
Glen said it had also engaged an auditor to examine issues.
Glen has to comply with the Regulator's direction by the end of this month.