Five people have been killed in four separate road accidents in Co Donegal since yesterday.
The victims range in age from 18 to 59.
Superintendent Vincent O'Brien of Letterkenny Garda Station said it seemed the road safety message is still not getting through in the county.
Up until yesterday road deaths had been down on the same period last year - three people had died compared to eight last year.
Now with five people killed this weekend alone, the number of road deaths in Donegal is the same as last year, eight.
The Donegal Road Safety Working Group issued a strong reminder to drivers in advance of the bank holiday weekend to drive with care.
The group has been running an awareness campaign launched by Gay Byrne which has the stated aim of saving lives and reducing serious injury on Donegal roads.
That campaign was carried through to yesterday's St Patrick's Day parade in Letterkenny, where the theme was road safety but Supt O'Brien says the message still does not seem to be getting through.
Five deaths on Co Donegal roads
19-year-old Roisin Doherty died in hospital after the car she was travelling in hit a tree earlier this morning.
She was a passenger in a car which crashed at Muff just after 3am. Anthony Doherty, 20 years, who was driving the car, died at the scene. Both were from Derry.
Earlier, 59-year-old Thomas Conaghan, from Mountcharles, was killed after the tractor he was driving left the road at Mountcharles and went into a field.
At 4am, Laura Hegarty, 18 years, from Letterkenny, died after being struck by a car at Newline Road in Letterkenny.
Gardaí have described the incident as a 'hit and run' and are appealing for anyone who may have information to contact them at Letterkenny Garda Station.
Yesterday, Martin McMullen, 20 years, from Dunfanaghy, Co Donegal, died after the car in which he was he was a front seat passenger lost control and collided with a wall at Derryart.
The 19-year-old driver of the car was taken to Letterkenny General Hospital with serious injuries.
His condition this morning was described as stable.