Amy Poehler and her husband will Arnett have split after nine years of marriage.
The Parks and Recreation star actress and the Arrested Development actor married in 2003 and have two sons together, 3-year-old Archie and 2-year-old Abel.
A representative for the couple confirmed the news to Us Weekly, with a source adding of the separation: "It's very amicable."
The pair have frequently starred in the same TV and film projects together, including Blades of Glory, Horton Hears a Who!, On Broadway, Spring Breakdown and Monsters vs. Aliens.
Arnett also guest starred in an episode of Poehler's NBC comedy Parks and Recreation in 2010.
He is currently working on the fourth season of Arrested Development, which will air on Netflix in 2013.
Parks and Recreation will return to NBC for its fifth season on September 20.