Britney Spears and Justin Timberlake made separate appearances on stage with Madonna on Thursday night in Los Angeles as part of her 'Sticky and Sweet' tour.

People.com reports that Spears took to the stage with Madonna to perform a version of the song 'Human Nature' from the singer's sixth album 'Bedtime Story'. Wearing black satin trousers and a white shirt, the singer appeared very relaxed. She has been performing the same song in a taped segment since Madonna began the tour.

After Britney’s appearance Justin Timberlake then joined Madonna on stage to perform '4 Minutes'.

Spears had two reasons to celebrate on Thursday night after she won the gongs for Act of 2008 and the Album of the Year for 'Blackout' at MTV’s European Music Awards.