Two-time champion Rafael Nadal was dumped out of Wimbledon by unsung Czech Lukas Rosol who described his triumph as "a miracle".
It was one of the most startling upsets the tournament has ever known.
Rosol lost the first set on a tie-break but refused to be swept aside by 11-time grand slam winner Nadal, and came back to win 6-7 (11/9) 6-4 6-4 2-6 6-4 - taking the decider under the Centre Court roof.
A 45-minute delay - initially scheduled to be just a half-hour break - preceded the fifth set, with officials sending the players back to the locker rooms to allow for the roof to be closed under fading light. The break worked in Rosol's favour, and he admitted the win over his fellow 26-year-old surpassed his wildest expectations.
He said: "It is a miracle for me - I never expected this.
"There are so many emotions. He is a superstar but I played unbelievable today. I hope I can play one more match like this.
"I am happy and congratulations to Rafa. He played a good match but I was better."
Spaniard Nadal admitted Rosol had outclassed him in the fifth set and deserved the win.
He said: "For sure [this match] wasn't the best one for me but that's what it is and I have to accept that he came back and played unbelievable in the fifth.
"I was playing well in the fourth - I think I played a great fourth set. Sure the stop this time didn't help me but that's the sport.
"You play against an inspired opponent and I am out. That's all. It is not a tragedy, it is only a tennis match."
Elsewhere, Croatian Ivo Karlovic bowed out and accused tournament officials of showing bias towards home favourite Andy Murray.
Big-serving Karlovic was seen off in four sets by Murray on Centre Court but was left seething about being repeatedly punished for foot faults.
One of the calls came in the fourth-set tie-break which Murray won to secure a 7-5 6-7 (7/5) 6-2 7-6 (7/4) victory.
It was an impressive performance from number four seed Murray whose route to the final is littered with potential hazards, but 6ft 10in Karlovic was in no mood to pat him on the back.
"In my whole life, ever since I was eight years old, I didn't do this many foot faults," he said.
"After I don't know how many, I stood a little bit back so they could not call it and they still did. It was outrageous. It's Wimbledon, Centre Court, and they do this. I feel cheated."
Today, top seed Novak Djokovic will look to move one step closer to retaining his title when he takes on Radek Stepanek in the third round while Roger Federer faces Julien Benneteau.
Spain's Nicolas Almagro tackles Richard Gasquet, Janko Tipsarevic plays Russian Mikhail Youzhny and Juan Monaco will attempt to beat Viktor Troicki.