The entire England squad are taking anti-malaria tablets ahead of their trip to Manaus, but Saturday's opponents Italy are going with a more laissez-faire approach.
England land in the Amazon on Thursday, while their Group D opponents arrive on the eve of the match.
England manager Roy Hodgson is not a fan of anti-malarials but insisted this week he had no option but to issue the pills to his players, despite the potentially damaging side-effects.
One in 10 people experience side-effects like stomach cramps, sickness and dizziness after taking the pills, which England have been taking daily since Tuesday morning.
There is no such diktat in the Italy camp, however, with the Italian Football Federation (FIGC) allowing players to make their own decision on whether to take the medication.
"We don't dictate anything to the players regarding this matter," a FIGC spokesman said on Wednesday.
"That is the responsibility of each player."
While England are taking anti-malaria tablets, they have decided not to have yellow fever jabs.