Paris St-Germain right-back Gregory van der Wiel was the most notable absentee from Holland coach Louis van Gaal's provisional World Cup squad, which was announced on Tuesday evening.
The 26-year-old defender had been enjoying a fine season for the Ligue 1 champions before suffering a knee injury that has put paid to his hopes of competing in Brazil.
There was no room either for Fulham pair John Heitinga and Maarten Stekelenburg, Barcelona midfielder Ibrahim Afellay, Stoke left-back Erik Pieters, AC Milan midfielder Urby Emanuelson and Ajax goalkeeper Kenneth Vermeer.
"It is always difficult to make choices, but we have made these choices for a reason," Van Gaal was quoted as saying at a press conference.
"Although it was difficult to tell the players who were not selected, they were good conversations. I got the impression that they handled it in a professional way.
"It is now up to those in the preliminary selection to convince me that they belong in the final 23."
The final 23-man list must be submitted to FIFA by Monday 2 June.
Holland provisional squad: Jasper Cillessen (Ajax), Tim Krul (Newcastle), Jeroen Zoet (PSV Eindhoven), Michel Vorm (Swansea); Patrick van Aanholt (Vitesse), Daley Blind (Ajax), Daryl Janmaat (Feyenoord), Terence Kongolo (Feyenoord), Bruno Martins Indi (Feyenoord), Karim Rekik (PSV Eindhoven), Joel Veltman (Ajax), Paul Verhaegh (Augsburg), Ron Vlaar (Aston Villa), Stefan de Vrij (Feyenoord); Jordy Clasie (Feyenoord), Leroy Fer (Norwich), Jonathan de Guzman (Swansea), Nigel de Jong (AC Milan), Wesley Sneijder (Galatasaray), Tonny Vilhena (Feyenoord), Rafael van der Vaart (Hamburg), Georginio Wijnaldum (PSV Eindhoven); Jean-Paul Boetius (Feyenoord), Memphis Depay (PSV Eindhoven), Klaas Jan Huntelaar (Schalke), Dirk Kuyt (Fenerbahce), Jeremain Lens (Dynamo Kiev), Robin van Persie (Manchester United), Quincy Promes (FC Twente), Arjen Robben (Bayern Munich).