Juventus coach Antonio Conte has failed in his bid to have a 10-month suspension from football overturned.
The 43-year-old was handed the lengthy ban by the Italian Football Federation (FIGC) for allegedly failing to report two incidents of match-fixing during his time in charge of Siena.
But despite appealing the verdict in person at the Federal Court of Justice on Monday, the 43-year-old has been denied the chance to return to coaching Juve for Saturday's opening Serie A game against Parma.
An FIGC statement read: "The Federal Court of Justice can confirm the 10-month disqualification for Antonio Conte."
Conte's implication in the Calcioscommesse scandal involved two Serie B matches that took place in the 2010-11 season.
The statement continued: "It was decided to acquit Conte for his role in the Novara-Siena game, but the 10-month disqualification stands as a disciplinary commission sanction concerning Albinoleffe-Siena."
Juventus President Andrea Agnelli said the ruling confirmed the club's "worst suspicions" about Italy's football justice system claiming it "seems more and more like a witch-hunt".
The FIGC's prosecutor also lost an appeal against the decision to acquit Juventus defender Leonardo Bonucci and winger Simone Pepe over an alleged attempt to fix a match between Bari and Udinese in 2010.
The FIGC's prosecutor had wanted a three-and-a-half year ban for Italy's Bonucci, who was playing for Bari at the time, and one-year ban for Pepe, who was with Udinese.
The Serie A season starts this weekend. Juve have said they will stick by Conte with a second appeal set to be launched and heard in September.
Conte's assistant Massimo Carrera has taken charge of Juve's pre-season friendlies and is poised to stay as temporary boss.