New Scotland head coach Vern Cotter has selected a total of 43 men for his first summer tour - including six uncapped players.
Glasgow forwards Gordon Reid, Alex Allan and Kevin Bryce and midfield colleague Finn Russell are joined by London Irish openside Blair Cowan and Edinburgh scrum-half Grayson Hart in seeking their international debuts in June.
Cotter has named separate squads for the North American and Southern Hemisphere legs of the tour but nine players have been included for the entire schedule.
There was no place for Glasgow captain Al Kellock or prop Euan Murray, who was recently released by Worcester.
Kellock recently returned from arm surgery but wingers Sean Lamont and Tim Visser are both included after making comebacks late in the season.
The nine players who will play a full part in the tour, which sees Scotland face the United States, Canada, Argentina and South Africa, are Reid, Pat MacArthur, Kevin Bryce, Moray Low, Geoff Cross and Grant Gilchrist and backs Alex Dunbar, Sean Maitland and Stuart Hogg.
Kyle Traynor will join the squad after Bristol's Greene King IPA Championship final second leg against London Welsh on June 4.
The Scottish Rugby Union revealed that Edinburgh pair Jack Cuthbert and Matt Scott and new Bristol signing Ross Rennie would have been included but for injury along with Glasgow forward Ryan Wilson, who was ruled out for several months with a shoulder injury on Monday.
The SRU also announced that a number of players would not tour because of elective surgery or possible involvement in the Commonwealth Games sevens tournament. Both Nick De Luca and Richie Vernon, who were called up for the recent Glasgow Sevens, are absent from the squad.
Cotter, who takes over from Scott Johnson, told www.scottishrugby.org: "I'm very much looking forward to starting work with the Scotland players as we begin our 16-match countdown to the 2015 Rugby World Cup in England.
"Working with Scott Johnson and the national coaches, we've selected, effectively, two squads for our itinerary this summer taking into account Glasgow Warriors' pursuit of Pro 12 silverware and the fact that our match against South Africa falls out-with the IRB Regulation 9 window.
"We've included a mix of the battle-hardened and promising new talent and it will be good to see how players step up to tough and different challenges at the end of a long domestic campaign."