Italy coach Jacques Brunel has called three uncapped players into his 32-man squad for the November test matches.
Fly-half Tommaso Allan and centre Michele Campagnaro were named in the national squad for the first time and winger Ruggero Trevisan has also been selected.
Paul Derbyshire, Quintin Geldenhuys, Andrea Masi and Francesco Minto were all ruled out by injury.
Forwards Martin Castrogiovanni, Marco Bortolami and Sergio Parisse are all in line to make their 100th appearances for Italy during the November series which starts against Australia and includes matches against Fiji and Argentina.
Forwards: Matias Aguero, Martin Castrogiovanni, Lorenzo Cittadini, Michele Rizzo, Leonardo Ghiraldini, Davide Giazzon, Andrea Manici, Valerio Bernabo, Marco Bortolami, Joshua Furno, Antonio Pavanello, Robert Barbieri, Mauro Bergamasco, Simone Favaro, Sergio Parisse (c), Manoa Vosawai, Alessandro Zanni
Backs: Tobias Botes, Alberto Chillon, Tommaso Allan, Alberto Di Bernado, Luciano Orquera, Michele Campagnaro, Gonzalo Canale, Gonzalo Garcia, Luca Morisi, Alberto Sgarbi, Tommaso Benvenuti, Tommaso Iannone, Luke McLean, Leonardo Sarto, Ruggero Trevisan, Alberto De Marchi, Edoardo Gori, Giovambattista Venditti