John Gosden's brilliant mare The Fugue has run her last race due to injury.
The five-year-old broke the track record in the Prince of Wales's Stakes at Royal Ascot only last month, beating last year's Breeders' Cup Turf winner Magician and Prix de l'Arc de Triomphe heroine Treve in the process.
She was sent off a hot favourite to follow up in the Coral-Eclipse at Sandown last Saturday but was always towards the rear of the field and never got involved, trailing home in sixth place.
Initial reports were that she had returned home unscathed but connections have been dealt a blow with the discovery of an injury to her near-fore.
Owned by Lord Lloyd Webber, the daughter of Dansili won six of her 17 starts, earning just short of £2million in win and place prize-money.
"When things fell right for her and she was on her A-game, she was very good"
Other career highlights included victories in the 2012 Nassau Stakes at Glorious Goodwood, when Richard Hughes rode as Buick opted for stablemate Izzi Top, and last season's Yorkshire Oaks and Irish Champion Stakes.
She was beaten half a length by Magician at Santa Anita and the following month was agonisingly denied again when three-quarters of a length behind Dominant in the Hong Kong Vase.
William Buick rode The Fugue to four of her victories and told Channel 4 Racing: "When things fell right for her and she was on her A-game, she was very good.
"She should [be] remembered for those good wins she had, especially in the Prince of Wales's [Stakes] this year. She broke the track record and beat world champion horses.
"She's retiring on a huge high. I know things didn't go right for her in the Eclipse and obviously the ground wasn't right.
"She did something to herself in the race and it explains why she was so under par.
"She lost nothing in defeat and she's filly that will always be close to my heart.
"She was all about pace - turn of foot, travel and pace.
"She always needed an uninterrupted run and sometimes things didn't go her way.
"She was a beautiful filly to ride, but it was always a fine line between getting it very right and getting it wrong with her.
"On her day, she was awesome. She was only out of the first three four times.
"She ran in America and has taken me all over the world.
"I'll never forget her, that's for sure."