Indian Chief now appears to head an eight-strong entry for the Betfred Dante Stakes at York on Thursday after the defection of Telescope.
The Galileo colt, owned by Highclere Thoroughbred Racing, was not among the final declarations because of a minor infection which developed in grazes on two of his legs.
The setback occurred when he was being loaded onto a horsebox, but the Derby on June 1 remains his objective.
Aidan O'Brien has carried all before him recently on the Derby trials front and his Indian Chief was impressive in winning a Leopardstown maiden last month.
In the absence of Telescope, Highclere rely solely on the Luca Cumani-trained Greatwood, who missed the Lingfield Derby Trial on Saturday due to rain-softened ground.
Kieren Fallon would not have been able to ride at the weekend due to suspension but is back in action on the Knavesmire.
Saeed bin Suroor seeks a third Dante success with Secret Number, who lost his unbeaten record when third to O'Brien's Lines Of Battle in the UAE Derby. He will be ridden by Silvestre de Sousa.
Mark Johnston's Windhoek (Joe Fanning) has already run twice this season, picking up a valuable sales prize at Newmarket - where he edged Greatwood - and following up back on the Rowley Mile in a Listed event.
Ghurair for John Gosden further represents that sales race at Newmarket as he was third that day, while Jim Bolger runs an interesting contender in Trading Leather, last seen finishing fifth in the Racing Post Trophy to Kingsbarns.
David Elsworth's Dashing Star and the Elaine Burke-trained Libertarian complete the line-up.