The Irish tandem of James Brown and Damien Shaw were forced to withdraw from the men’s individual B road race when they suffered a puncture followed by a mechanical in the first 32km of the 104km race.
Before their withdrawal, the duo were active at the front of the race, working with the Beijing Paralympic champions Poland, and reigning world champions France.
Brown and Shaw have only been together since January, and have been moving from strength to strength, winning a bronze in the time-trial earlier this week, and narrowly missing out on a medal in the pursuit at the Velodrome.
Both riders combine training at this elite level with full-time jobs, and despite this disadvantage have been producing outstanding results.
After the race, Shaw said: “We were going well, riding in a good position and feeling strong.
"When you are riding on tubular tyres they are fast, but they can puncture a bit easier, which is what happened.
"It took a long time to change, the support cars don’t follow the race which makes it difficult."
Brown added: "The road race is a bit of a lottery, we had a plan, but you can’t predict what will happen out there.
"We are lucky we didn’t puncture in the time trial."
In this morning's women's individual B road race, Katie Dunlevey and her pilot Sandra Fitzgerald finished fifth for the third time in these Paralympic Games with a time of 2:12:56.
Catherine Walsh and her pilot Fran Meehan finished ninth in a time of 2:13:04.