Winner Anacona claimed victory on stage nine of the Vuelta a Espana as Nairo Quintana inherited the red jersey and Chris Froome lost time in the general classification.
The overall standings ahead of the first rest day see previous front-runner Alejandro Valverde (Movistar) having slipped to third, eight seconds off the top, and fellow Spaniard Alberto Contador (Tinkoff-Saxo) up to second, three seconds behind new leader Quintana.
Colombia's Anacona (Lampre-Merida) is fourth, trailing compatriot Quintana (Movistar) by nine seconds, while Briton Froome (Team Sky) - 20 seconds off the lead on Saturday - has dropped a place to fifth and is now 28 seconds back.
Anacona was part of a large breakaway that built up a healthy advantage over the peloton, and the Lampre-Merida man made his move off the front in a bid to put himself back in the general classification picture after losing time earlier in the race.
And he did just that, finishing more than two minutes ahead of the top names to slot in to fourth place overall, nine seconds back from Quintana after Sunday's stage, which ran a total of 185km from Carboneras de Guadazaon to Aramon Valdelinares.
Ireland’s Dan Martin (Garmin Sharp) finished in 20th and is now 15th in the general classification, while compatriot Philip Deignan was 64th and lies 55th overall.