Members of the Irish Nurses and Midwives Organisation (INMO) at St Vincent's University Hospital (SVUH), Dublin, will engage in a lunchtime protest tomorrow.
The protest will take place from 1-2pm outside the hospital.
INMO Industrial Relations Officer Philip McAnenly said: "At a meeting this evening, INMO members expressed their frustration with the chronic shortage of nursing posts at St Vincent's Hospital.
"They are increasingly concerned at the position they find themselves in on a daily basis attempting to deliver safe care," Mr McAnenly said.
"Nurses now call on hospital management to work with them, to immediately curtail services to a safe level and to exhaust all possible options of patient care including referring patients to St Colmcille's Hospital in Loughlinstown and St Michael's Hospital in Dun Laoghaire.
"INMO members are extremely disappointed that they now have to publically protest on this issue less than a month after the ED Agreement was brokered," he said.
An INMO statement said: "The protest on Wednesday 2 March 2016 is the commencement of a campaign to ensure that SVUH management respond to the urgent concerns of patients and staff."