Olivia Agbonlahor has lost her battle against deportation and will leave Ireland for Nigeria with her two children this evening.
Ms Agbonlahor, her autistic son Great and his twin sister Melissa, have been living in Killarney for the past five years.
In a letter from the Department of Justice last month, they were granted a stay of four weeks on their original deportation date.
The children were given inoculation shots and the delay allowed time for the medication to take effect.
Six-year-old Great has been receiving one-to-one treatment for his autism in Killarney, which was said to be proving effective.
Supporters staged demonstrations at the Immigration Bureau and appealed to the Minister for Justice, Brian Lenihan, to reverse the decision.
They said that when Great is deported he will face a life without treatment in a society where autism is not understood.