Last week Madonna surprised fans during a performance of Human Nature at the Turk Telecom Arena in Istanbul by revealing her right breast to the audience.

While performing her 1995 hit last Thursday night, the 53-year-old revealed more than expected to the 55,000 in attendance at the gig, reports the Daily Mail.

The singer was performing wearing a pair of pin striped trousers and a white shirt which removed mid-tune. She then removed the first layer of the bra before going on to pull one cup down revealing her breast.

The singer recently started her World tour in Israel and arrived in Turkey last Tuesday. Her next performance of the MDNA tour will be in Rome’s Stadio Olimpico tomorrow evening.

Jean-Paul Gaultier has created the wardrobe for the entire tour and Madonna has praised him saying "he's a genius", the French designer has even updated her iconic cone bra outfit.